Why were the cells placed in CaCl2 and heat shock during this experiment?
Why were the cells placed in CaCl2 and heat shock during this experiment? The heat shock step strongly depolarizes the cell membrane of CaCl2-treated cells. Thus, the decrease in membrane potential lowers the negativity of the cell’s inside potential which ultimately allows the movement of negatively charged DNA into the cell’s interior. How does CaCl2 … Continue reading Why were the cells placed in CaCl2 and heat shock during this experiment?
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